Two probing strategies have generally been employed, namely, dot-blot hybridization and fluorescent in situ hybridization. 兩種研究方法被普遍采用，即斑點(diǎn)雜交法和熒光原位雜交。
Plasmid DNA and chromosomal DNA of the strain CEOI all obtained positive dot-blot to plasmid probe and chromosomal probe correspondingly. 質(zhì)粒DNA和染色體DNA分別與質(zhì)粒探針和染色體探針雜交出現(xiàn)典型的陽性雜交斑。
Comparison of the two methods of dot-blot hybridization and PCR showed that the latter was more sensitive than the former, which indicated the replication of DHBV was low in the tissues. 進(jìn)一步比較了斑點(diǎn)雜交法和PCR法檢測的結(jié)果，顯示斑點(diǎn)雜交法檢測組織中病毒遠(yuǎn)不如PCR法敏感，并提示可能組織中病毒復(fù)制量很低，以致用斑點(diǎn)雜交法檢測出現(xiàn)很多假陰性。